Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons.

ABSTRACT: The differentiation of neuronal stem cells into polarized neurons is a well-coordinated process which has mostly been studied in classical non-human model systems, but to what extent these findings are recapitulated in human neurons remains unclear. To study neuronal polarization in human neurons, we cultured hiPSC-derived neurons, characterized early developmental stages, measured electrophysiological responses, and systematically profiled transcriptomic and proteomic dynamics during these steps. The neuron transcriptome and proteome shows extensive remodeling, with differential expression profiles of ~1100 transcripts and ~2200 proteins during neuronal differentiation and polarization. We also identified a distinct axon developmental stage marked by the relocation of axon initial segment

SUBMITTER: Lindhout FW 

PROVIDER: S-EPMC7498259 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature