[Detection of the ermB gene associated with macrolide resistance in Campylobacter strains isolated from chickens marketed in Lima, PeruDeteccao do gene ermB associado a resistencia a macrolideos em cepas de Campylobacter isoladas de frangos comercializados em Lima, no Peru].

ABSTRACT:

Objective

To detect the presence of the ermB gene associated with macrolide resistance in Campylobacter spp. strains isolated from chickens marketed in Lima, Peru.

Methods

120 samples of chicken skin from three markets in the districts of San Martin de Porres (n = 30), Santa Anita (n = 20), and Independencia (n = 70), located in the Province of Lima, Peru, were analyzed. Microbiological analysis of the samples was carried out according to ISO standard 10272-1:2017. For the polymerase chain reaction (PCR) confirmation of genus and species, 16-rRNA and GlyA and hipO primers, respectively, were used. For the evaluation of antibiotic sensitivity, the Müller-Hinton agar with 5% blood, with sensi-discs for azithromycin (15 µg) and erythromycin (15 µg), was used. For

SUBMITTER: Anampa D 

PROVIDER: S-EPMC7498294 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature