IMPLICON: an ultra-deep sequencing method to uncover DNA methylation at imprinted regions.

ABSTRACT: Genomic imprinting is an epigenetic phenomenon leading to parental allele-specific expression. Dosage of imprinted genes is crucial for normal development and its dysregulation accounts for several human disorders. This unusual expression pattern is mostly dictated by differences in DNA methylation between parental alleles at specific regulatory elements known as imprinting control regions (ICRs). Although several approaches can be used for methylation inspection, we lack an easy and cost-effective method to simultaneously measure DNA methylation at multiple imprinted regions. Here, we present IMPLICON, a high-throughput method measuring DNA methylation levels at imprinted regions with base-pair resolution and over 1000-fold coverage. We adapted amplicon bisulfite-sequencing protocols to d

SUBMITTER: Klobucar T 

PROVIDER: S-EPMC7498334 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature