DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions.

ABSTRACT: Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that DropSynth can build thousands of gene-length fragments at >20% fidelity.

SUBMITTER: Sidore AM 

PROVIDER: S-EPMC7498354 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature