Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta cells.

ABSTRACT:

Objectives

The main endocrine cell types in pancreatic islets are alpha, beta, and delta cells. Although these cell types have distinct roles in the regulation of glucose homeostasis, inadequate purification methods preclude the study of cell type-specific effects. We developed a reliable approach that enables simultaneous sorting of live alpha, beta, and delta cells from mouse islets for downstream analyses.

Methods

We developed an antibody panel against cell surface antigens to enable isolation of highly purified endocrine subsets from mouse islets based on the specific differential expression of CD71 on beta cells and CD24 on delta cells. We rigorously demonstrated the reliability and validity of our approach using bulk and single cell qPCR, immunocytochemistry, reporter

SUBMITTER: Berthault C 

PROVIDER: S-EPMC7498953 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature