Extracellular Vesicles Produced by Bifidobacterium longum Export Mucin-Binding Proteins.

ABSTRACT: Extracellular proteins are important factors in host-microbe interactions; however, the specific factors that enable bifidobacterial adhesion and survival in the gastrointestinal (GI) tract are not fully characterized. Here, we discovered that Bifidobacterium longum NCC2705 cultured in bacterium-free supernatants of human fecal fermentation broth released a myriad of particles into the extracellular environment. The aim of this study was to characterize the physiological properties of these extracellular particles. The particles, approximately 50 to 80 nm in diameter, had high protein and double-stranded DNA contents, suggesting that they were extracellular vesicles (EVs). A proteomic analysis showed that the EVs primarily consisted of cytoplasmic proteins with crucial functions in essenti

SUBMITTER: Nishiyama K 

PROVIDER: S-EPMC7499026 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature