Quantifying Temperature Compensation of Bicoid Gradients with a Fast T-Tunable Microfluidic Device.

ABSTRACT: As a reaction-diffusion system strongly affected by temperature, early fly embryos surprisingly show highly reproducible and accurate developmental patterns during embryogenesis under temperature perturbations. To reveal the underlying temperature compensation mechanism, it is important to overcome the challenge in quantitative imaging on fly embryos under temperature perturbations. Inspired by microfluidics generating temperature steps on fly embryos, here we design a microfluidic device capable of ensuring the normal development of multiple fly embryos as well as achieving real-time temperature control and fast temperature switches for quantitative live imaging with a home-built two-photon microscope. We apply this system to quantify the temperature compensation of the morphogen Bicoid (

SUBMITTER: Zhu H 

PROVIDER: S-EPMC7499060 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature