In vivo localization of chronically implanted electrodes and optic fibers in mice.

ABSTRACT: Electrophysiology provides a direct readout of neuronal activity at a temporal precision only limited by the sampling rate. However, interrogating deep brain structures, implanting multiple targets or aiming at unusual angles still poses significant challenges for operators, and errors are only discovered by post-hoc histological reconstruction. Here, we propose a method combining the high-resolution information about bone landmarks provided by micro-CT scanning with the soft tissue contrast of the MRI, which allowed us to precisely localize electrodes and optic fibers in mice in vivo. This enables arbitrating the success of implantation directly after surgery with a precision comparable to gold standard histology. Adjustment of the recording depth with micro-drives or early termination of

SUBMITTER: Kiraly B 

PROVIDER: S-EPMC7499215 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature