A fluorescent ESIPT-based benzimidazole platform for the ratiometric two-photon imaging of ONOO^- in vitro and ex vivo.

ABSTRACT: In this work, we have developed an ESIPT-based benzimidazole platform (MO-E1 and MO-E2) for the two-photon cell imaging of ONOO^- and a potential ONOO^--activated theranostic scaffold (MO-E3). Each benzimidazole platform, MO-E1-3, were shown to rapidly detect ONOO^- at micromolar concentrations (LoD = 0.28 μM, 6.53 μM and 0.81 μM respectively). The potential theranostic MO-E3 was shown to release the parent fluorophore and drug indomethacin in the presence of ONOO^- but unfortunately did not perform well in vitro due to low solubility. Despite this, the parent scaffold MO-E2 demonstrated its effectiveness as a two-photon imaging tool for the ratiometric detection of endogenous ONOO^- in RAW26

SUBMITTER: Odyniec ML 

PROVIDER: S-EPMC7499849 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature