A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.

ABSTRACT:

Objective

Owing to the overwhelming dominance of human and commensal microbe sequences, low efficiency is a major concern in clinical viral sequencing using next-generation sequencing. DNA composed of 7-deaza-2'-deoxyguanosine 5'-triphosphate (c⁷dGTP), an analog of deoxyguanosine triphosphate (dGTP), is resistant to selective restriction enzymes. This characteristic has been utilized to develop a novel strategy for target enrichment in next-generation sequencing.

Results

The new enrichment strategy is named target enrichment via enzymatic digestion in next-generation sequencing (TEEDseq). It combined 7-deaza-2'-deoxyguanosine 5'-triphosphate (c⁷dGTP)-involved primer extension, splinter-assisted intracellular cyclization, c⁷dGTP)-resistant enz

SUBMITTER: Peng P 

PROVIDER: S-EPMC7499927 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature