Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus.

ABSTRACT:

Background

Reference genes are usually stably expressed in various cells and tissues. However, it was reported that the expression of some reference genes may be distinct in different species. In this study, we intend to answer whether the expression of reported traditional reference genes changes or not in the polyploid fish RESULTS: By retrieving the mRNA sequencing data of three different ploidy fish from the NCBI SRA database, we selected 12 candidate reference genes, and examined their expression levels in the 10 tissues and in the four cell lines of three different ploidy fish by real-time PCR. Then, the expression profiles of these 12 candidate reference genes were systematically evaluated by using the software platforms: BestKeeper, NormFinder and geNorm.

Conclusion

SUBMITTER: Liu W 

PROVIDER: S-EPMC7499967 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature