CLPTM1L induces estrogen receptor β signaling-mediated radioresistance in non-small cell lung cancer cells.

ABSTRACT:

Introduction

Radioresistance is a major challenge in lung cancer radiotherapy, and new radiosensitizers are urgently needed. Estrogen receptor β (ERβ) is involved in the progression of non-small cell lung cancer (NSCLC), however, the role of ERβ in the response to radiotherapy in lung cancer remains elusive. In the present study, we investigated the mechanism underlying ERβ-mediated transcriptional activation and radioresistance of NSCLC cells.

Methods

Quantitative real-time PCR, western blot and immunohistochemistry were used to detect the expression of CLPTM1L, ERβ and other target genes. The mechanism of CLPTM1L in modulation of radiosensitivity was investigated by chromatin immunoprecipitation assay, luciferase reporter gene assay, immunofluorescence staining, confocal m

SUBMITTER: Li H 

PROVIDER: S-EPMC7499972 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature