Noninvasive Imaging of Cone Ablation and Regeneration in Zebrafish.

ABSTRACT:

Purpose

To observe and characterize cone degeneration and regeneration in a selective metronidazole-mediated ablation model of ultraviolet-sensitive (UV) cones in zebrafish using in vivo optical coherence tomography (OCT) imaging.

Methods

Twenty-six sws1:nfsB-mCherry;sws2:eGFP zebrafish were imaged with OCT, treated with metronidazole to selectively kill UV cones, and imaged at 1, 3, 7, 14, 28, or 56 days after ablation. Regions 200 × 200 µm were cropped from volume OCT scans to count individual UV cones before and after ablation. Fish eyes were fixed, and immunofluorescence staining was used to corroborate cone density measured from OCT and to track monocyte response.

Results

Histology shows significant loss of UV cones after metronidazole treatment with a sli

SUBMITTER: Huckenpahler AL 

PROVIDER: S-EPMC7500127 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature