Enhanced lipid biosynthesis in human tumor-induced macrophages contributes to their protumoral characteristics.

ABSTRACT:

Background

Tumor-associated macrophages (TAMs) are key components of the tumor microenvironment (TME) in non-medullary thyroid carcinoma (TC) and neuroblastoma (NB), being associated with a poor prognosis for patients. However, little is known about how tumors steer the specific metabolic phenotype and function of TAMs.

Methods

In a human coculture model, transcriptome, metabolome and lipidome analysis were performed on TC-induced and NB-induced macrophages. The metabolic shift was correlated to functional readouts, such as cytokine production and reactive oxygen species (ROS) production, including pharmacological inhibition of metabolic pathways.

Results

Based on transcriptome and metabolome analysis, we observed a strong upregulation of lipid biosynthesis pathways i

SUBMITTER: Rabold K 

PROVIDER: S-EPMC7500191 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature