PH dependence of the Slc4a11-mediated H⁺ conductance is influenced by intracellular lysine residues and modified by disease-linked mutations.

ABSTRACT: SLC4A11 is the only member of the SLC4 family that transports protons rather than bicarbonate. SLC4A11 is expressed in corneal endothelial cells, and its mutation causes corneal endothelial dystrophy, although the mechanism of pathogenesis is unknown. We previously demonstrated that the magnitude of the H⁺ conductance (G_m) mediated by SLC4A11 is increased by rises in intracellular as well as extracellular pH (pH_i and pH_e). To better understand this feature and whether it is altered in disease, we studied the pH dependence of wild-type and mutant mouse Slc4a11 expressed in Xenopus oocytes. Using voltage-clamp circuitry in conjunction with a H⁺-selective microelectrode and a microinjector loaded with NaHCO₃, we c

SUBMITTER: Quade BN 

PROVIDER: S-EPMC7500215 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature