A smartphone microscopic method for simultaneous detection of (oo)cysts of Cryptosporidium and Giardia.

ABSTRACT:

Background

Food and water-borne illness caused by ingestion of (oo)cysts of Cryptosporidium and Giardia is one of the major health problems globally. Several methods are available to detect Giardia cyst and Cryptosporidium oocyst in food and water. Most of the available methods require a good laboratory facility and well-trained manpower and are therefore costly. There is a need of affordable and reliable method that can be easily implemented in resource limited settings.

Methodology/principle findings

We developed a smartphone based microscopic assay method to screen (oo)cysts of Cryptosporidium and Giardia contamination of vegetable and water samples. The method consisting of a ball lens of 1 mm diameter, white LED as illumination source and Lugols's iodine staining provid

SUBMITTER: Shrestha R 

PROVIDER: S-EPMC7500626 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature