Proteolytic profile of larval developmental stages of Penaeus vannamei: An activity and mRNA expression approach.

ABSTRACT: In arthropods, the cleavage of specific proteins by peptidases has pivotal roles in multiple physiological processes including oogenesis, immunity, nutrition, and parasitic infection. These enzymes are also key players in the larval development, and well-described triggers of molting and metamorphosis. In this work the peptidase complement throughout the larvae development of Penaeus vannamei was quantified at the transcript and activity level using qPCR and fluorogenic substrates designed to be hydrolyzed by class-specific peptidases respectively, providing a detailed identification of the proteolytic repertoire in P. vannamei larvae. Significant changes in the peptidase activity profile were observed. During the lecithotrophic naupliar instars, the dominant peptidase activity and express

SUBMITTER: Rojo-Arreola L 

PROVIDER: S-EPMC7500676 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature