Neuroprotective effects of Camellia nitidissima Chi leaf extract in hydrogen peroxide-treated human neuroblastoma cells and its molecule mechanisms.

ABSTRACT: Camellia nitidissima Chi (CNC) is a famous medicinal and edible plant with the name of "Tea for Longevity" in Guangxi province of China. In present study, we determined the protective effect of extract from CNC leaves on H₂O₂-induced cell injury and its underlying mechanisms in human neuroblastoma (SH-SY5Y) cells. The ethyl acetate fraction of CNC leaves (CLE, 50-200 μg/ml) treatment significantly increased the cell viability of H₂O₂-treated SH-SY5Y cells and reduced the leakage of LDH in a reversed "U"-shape manner. It was confirmed by Hoechst 33,342 staining that CLE attenuated H₂O₂-induced apoptosis in SH-SY5Y cells. The CLE (100 and 150 μg/ml) treatment significantly relieved H₂O₂-induced oxi

SUBMITTER: An L 

PROVIDER: S-EPMC7500780 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature