Tonic TCR Signaling Inversely Regulates the Basal Metabolism of CD4⁺ T Cells.

ABSTRACT: The contribution of self-peptide-MHC signaling in CD4⁺ T cells to metabolic programming has not been definitively established. In this study, we employed LLO118 and LLO56, two TCRtg CD4⁺ T cells that recognize the same Listeria epitope. We previously have shown that LLO56 T cells are highly self-reactive and respond poorly in a primary infection, whereas LLO118 cells, which are less self-reactive, respond well during primary infection. We performed metabolic profiling and found that naive LLO118 had a dramatically higher basal respiration rate, a higher maximal respiration rate, and a higher glycolytic rate relative to LLO56. The LLO118 cells also exhibited a greater uptake of 2-NBD-glucose, in vitro and in vivo. We extended the correlation of low self-reactivi

SUBMITTER: Milam AAV 

PROVIDER: S-EPMC7500861 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature