TbRAP1 has an unusual duplex DNA binding activity required for its telomere localization and VSG silencing.

ABSTRACT: Localization of Repressor Activator Protein 1 (RAP1) to the telomere is essential for its telomeric functions. RAP1 homologs either directly bind the duplex telomere DNA or interact with telomere-binding proteins. We find that Trypanosoma brucei RAP1 relies on a unique double-stranded DNA (dsDNA) binding activity to achieve this goal. T. brucei causes human sleeping sickness and regularly switches its major surface antigen, variant surface glycoprotein (VSG), to evade the host immune response. VSGs are monoallelically expressed from subtelomeres, and TbRAP1 is essential for VSG regulation. We identify dsDNA and single-stranded DNA binding activities in TbRAP1, which require positively charged ₇₃₇RKRRR₇₄₁ residues that overlap with Tb

SUBMITTER: Afrin M 

PROVIDER: S-EPMC7500927 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature