DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients.

ABSTRACT:

Objective

This study aimed to highlight the importance of mutations within Proteus mirabilis genome that are related to fluoroquinolone resistance.

Methods

This is a cross sectional study performed in different teaching hospitals in Khartoum State from June 2016 to May 2017. A total of (120) P mirabilis isolates from patients with symptoms of UTIs attending different hospitals in Khartoum State were examined. First, modified Kurby Bauer method was performed for phenotypical detection of resistant isolates. Then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by sequencing were applied for detection of mutations in GyrA, GyrB, ParC and ParE genes of isolates.

Results

P. mirabilis sh

SUBMITTER: Abdelkreem RH 

PROVIDER: S-EPMC7501041 | biostudies-literature | 2020 Sep-Oct

REPOSITORIES: biostudies-literature