Autophagy induction promotes renal cyst growth in polycystic kidney disease.

ABSTRACT:

Background

Polycystic kidney disease (PKD) involves renal cysts arising from proliferating tubular cells. Autophagy has been recently suggested as a potential therapeutic target in PKD, and mammalian target of rapamycin (mTOR) is a key negative regulator of autophagy. However, the effect of autophagy regulation on cystogenesis has not been elucidated in PKD mice.

Methods

Clinical validation was performed using GEO datasets and autosomal dominant polycystic kidney disease (ADPKD) patient samples. Newly established PKD and LC3 transgenic mice were used for in vivo verifications, and additional tests were performed in vitro and in vivo using multiple autophagy drugs.

Findings

Neither autophagy stimulation nor LC3 overexpression alleviated PKD. Furthermore, we observed th

SUBMITTER: Lee EJ 

PROVIDER: S-EPMC7501056 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature