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Xist attenuates acute inflammatory response by female cells.

ABSTRACT: Biological sex influences inflammatory response, as there is a greater incidence of acute inflammation in men and chronic inflammation in women. Here, we report that acute inflammation is attenuated by X-inactive specific transcript (Xist), a female cell-specific nuclear long noncoding RNA crucial for X-chromosome inactivation. Lipopolysaccharide-mediated acute inflammation increased Xist levels in the cytoplasm of female mouse J774A.1 macrophages and human AML193 monocytes. In both cell types, cytoplasmic Xist colocalizes with the p65 subunit of NF-κB. This interaction was associated with reduced NF-κB nuclear migration, suggesting a novel mechanism to suppress acute inflammation. Further supporting this hypothesis, expression of 5' XIST in male cells significantly reduced IL-6 and NF-κB activity. Adoptive transfer of male splenocytes expressing Xist reduced acute paw swelling in male mice indicating that Xist can have a protective anti-inflammatory effect. These findings show that XIST has functions beyond X chromosome inactivation and suggest that XIST can contribute to sex-specific differences underlying inflammatory response by attenuating acute inflammation in women.

SUBMITTER: Shenoda BB

PROVIDER: S-EPMC7501270 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Xist attenuates acute inflammatory response by female cells.

Shenoda Botros B BB Ramanathan Sujay S Gupta Richa R Tian Yuzhen Y Jean-Toussaint Renee R Alexander Guillermo M GM Addya Sankar S Somarowthu Srinivas S Sacan Ahmet A Ajit Seena K SK

Cellular and molecular life sciences : CMLS 20200319 1

Biological sex influences inflammatory response, as there is a greater incidence of acute inflammation in men and chronic inflammation in women. Here, we report that acute inflammation is attenuated by X-inactive specific transcript (Xist), a female cell-specific nuclear long noncoding RNA crucial for X-chromosome inactivation. Lipopolysaccharide-mediated acute inflammation increased Xist levels in the cytoplasm of female mouse J774A.1 macrophages and human AML193 monocytes. In both cell types, ...[more]

PMID: 32193609

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Xist attenuates acute inflammatory response by female cells.

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Xist attenuates acute inflammatory response by female cells.

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