Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct.

ABSTRACT: Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient transfection of adherent 293T cells with multiple plasmids. We report here a method to rapidly generate such producer cell lines from 293T cells by stable transfection of a single DNA construct encoding all lentiviral vector components. The resulting suspension cell lines yield titers as high as can be achieved with transient transfection, can be readily scaled up in single-use stirred-tank bioreactors, and are genetically and functionally stable in extended cell culture. By removing the requirement for efficient transient transfection during upstrea

SUBMITTER: Chen YH 

PROVIDER: S-EPMC7501408 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature