Dynamics of the transcriptome during chicken embryo development based on primordial germ cells.

ABSTRACT:

Objective

Regulation of gene expression during embryo development on the basis of migration of primordial germ cells (PGCs) in vivo has been rarely studied due to limited cell number and the necessity to isolate PGCs from a large number of embryos. Moreover, little is known about the comprehensive dynamics of the transcriptome in chicken PGCs during early developmental stages. The current study investigated transcriptome dynamics of chicken PGCs at key developmental stages: 4.5, 8 and 12 days of embryo incubation. PGCs were collected, and RNA was isolated using a commercial kit for single cells. The isolated RNA was subjected to microarray analysis (Agilent Technologies).

Results

Between 8 and 12 days of incubation, the highest number of genes was regulated. These data indic

SUBMITTER: Dunislawska A 

PROVIDER: S-EPMC7501632 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature