Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord.

ABSTRACT: In the central nervous system, hyperpolarization-activated, cyclic nucleotide-gated (HCN1-4) channels have been implicated in neuronal excitability and synaptic transmission. It has been reported that HCN channels are expressed in the spinal cord, but knowledge about their physiological roles, as well as their distribution profiles, appear to be limited. We generated a transgenic mouse in which the expression of HCN4 can be reversibly knocked down using a genetic tetracycline-dependent switch and conducted genetically validated immunohistochemistry for HCN4. We found that the somata of HCN4-immunoreactive (IR) cells were largely restricted to the ventral part of the inner lamina II and lamina III. Many of these cells were either parvalbumin- or protein kinase Cγ (PKCγ)-IR. By using two dif

SUBMITTER: Nakagawa T 

PROVIDER: S-EPMC7501643 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature