Development of a CD19 PET tracer for detecting B cells in a mouse model of multiple sclerosis.

ABSTRACT:

Background

B cells play a central role in multiple sclerosis (MS) through production of injurious antibodies, secretion of pro-inflammatory cytokines, and antigen presentation. The therapeutic success of monoclonal antibodies (mAbs) targeting B cells in some but not all individuals suffering from MS highlights the need for a method to stratify patients and monitor response to treatments in real-time. Herein, we describe the development of the first CD19 positron emission tomography (PET) tracer, and its evaluation in a rodent model of MS, experimental autoimmune encephalomyelitis (EAE).

Methods

Female C57BL/6 J mice were induced with EAE through immunization with myelin oligodendrocyte glycoprotein (MOG_1-125). PET imaging of naïve and EAE mice was performed 19 h a

SUBMITTER: Stevens MY 

PROVIDER: S-EPMC7501720 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature