Microbially competent 3D skin: a test system that reveals insight into host-microbe interactions and their potential toxicological impact.

ABSTRACT: The skin`s microbiome is predominantly commensalic, harbouring a metabolic potential far exceeding that of its host. While there is clear evidence that bacteria-dependent metabolism of pollutants modulates the toxicity for the host there is still a lack of models for investigating causality of microbiome-associated pathophysiology or toxicity. We now report on a biologically characterised microbial-skin tissue co-culture that allows studying microbe-host interactions for extended periods of time in situ. The system is based on a commercially available 3D skin model. In a proof-of-concept, this model was colonised with single and mixed cultures of two selected skin commensals. Two different methods were used to quantify the bacteria on the surface of the skin models. While Micrococcus luteu

SUBMITTER: Lemoine L 

PROVIDER: S-EPMC7502063 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature