Development of three multiplex-PCR assays for virulence profiling of different iron acquisition systems in Escherichia coli.

ABSTRACT:

Background and objectives

Escherichia coli is responsible for various enteric and extraintestinal infections in animals and humans. Iron as an essential nutrient, has a proven role in pathogenicity of E. coli. Pathogenic E. coli benefits of having complicated systems for iron acquisition but our current knowledge is limited because of complexity of these systems. In the present study, three multiplex-PCR assays were developed to screen nine different virulence genes related to diverse iron acquisition systems in E. coli.

Materials and methods

The multiplex-PCR systems were designed and optimized in three panels. Each panel includes a triplex-PCR cocktail. The panels are as follow: panel 1: iroN, iutA and fecA; panel 2: fyuA, sitA<

SUBMITTER: Rahmani HK 

PROVIDER: S-EPMC7502150 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature