Impact of the Endosomal Escape Activity of Cell-Penetrating Peptides on the Endocytic Pathway.

ABSTRACT: Cell-penetrating peptides (CPPs) are routinely used for the delivery of macromolecules into live human cells. To enter the cytosolic space of cells, CPPs typically permeabilize the membrane of endosomes. In turn, several approaches have been developed to increase the endosomal membrane permeation activity of CPPs so as to improve delivery efficiencies. The endocytic pathway is, however, important in maintaining cellular homeostasis, and understanding how endosomal permeation impacts cells is now critical to define the general utility of CPPs. Herein, we investigate how CPP-based delivery protocols affect the endocytic network. We detect that, in some cases, cell penetration induces the activation of Chmp1b, Galectin-3, and TFEB, which are components of endosomal repair, organelle clearance

SUBMITTER: Kondow-McConaghy HM 

PROVIDER: S-EPMC7502533 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature