Developing a population-state decision system for intelligently reprogramming extracellular electron transfer in Shewanella oneidensis.

ABSTRACT: The unique extracellular electron transfer (EET) ability has positioned electroactive bacteria (EAB) as a major class of cellular chassis for genetic engineering aimed at favorable environmental, energy, and geoscience applications. However, previous efforts to genetically enhance EET ability have often impaired the basal metabolism and cellular growth due to the competition for the limited cellular resource. Here, we design a quorum sensing-based population-state decision (PSD) system for intelligently reprogramming the EET regulation system, which allows the rebalanced allocation of the cellular resource upon the bacterial growth state. We demonstrate that the electron output from Shewanella oneidensis MR-1 could be greatly enhanced by the PSD system via shifting the dominant meta

SUBMITTER: Li FH 

PROVIDER: S-EPMC7502708 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature