Direct kinetic fingerprinting and digital counting of single protein molecules.

ABSTRACT: The sensitive and accurate quantification of protein biomarkers plays important roles in clinical diagnostics and biomedical research. Sandwich ELISA and its variants accomplish the capture and detection of a target protein via two antibodies that tightly bind at least two distinct epitopes of the same antigen and have been the gold standard for sensitive protein quantitation for decades. However, existing antibody-based assays cannot distinguish between signal arising from specific binding to the protein of interest and nonspecific binding to assay surfaces or matrix components, resulting in significant background signal even in the absence of the analyte. As a result, they generally do not achieve single-molecule sensitivity, and they require two high-affinity antibodies as well as strin

SUBMITTER: Chatterjee T 

PROVIDER: S-EPMC7502736 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature