Analysis of β₂AR-G_s and β₂AR-G_i complex formation by NMR spectroscopy.

ABSTRACT: The β₂-adrenergic receptor (β₂AR) is a prototypical G protein-coupled receptor (GPCR) that preferentially couples to the stimulatory G protein G_s and stimulates cAMP formation. Functional studies have shown that the β₂AR also couples to inhibitory G protein G_i, activation of which inhibits cAMP formation [R. P. Xiao, Sci. STKE 2001, re15 (2001)]. A crystal structure of the β₂AR-G_s complex revealed the interaction interface of β₂AR-G_s and structural changes upon complex formation [S. G. Rasmussen et al., Nature 477, 549-555 (2011)], yet, the dynamic process of the β₂AR signaling through G_s and its preferential coupling to G_s over G_i is still n

SUBMITTER: Ma X 

PROVIDER: S-EPMC7502740 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature