Dataset Information

Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant Staphylococcus aureus.

ABSTRACT: Biofilms are multi-species bacterial communities with complex structures that create antibiotic resistance, cause life-threatening infections, thereby considerable economic loss; needed new approaches. Medicinal plants are focused as new alternatives for their therapeutic and antimicrobial effects. Our present study, Azadirachta indica, Moringa oleifera, Murraya koenigii, and Psidium guajava extracts were investigated against MRSA. The preliminary antimicrobial study showed pet. ether extract of A. indica and ethanolic extract of P. guajava showed a MIC value of 125 μg/mL and MBC value of 500 μg/mL. These extracts showed biofilm inhibition in the range of 60.0-83.9 % and did not possess any hemolytic activity to the human erythrocytes. The plant species investigated in this study had different degrees of antibiofilm activity against MRSA. However, we suggest that A. indica and P. guajava are promising candidates and further investigation is needed to isolate the antimicrobial compounds for the management of MRSA and its mechanism of activity.

SUBMITTER: S LP

PROVIDER: S-EPMC7502823 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant Staphylococcus aureus.

S Lakshmana Prabu LP A Umamaheswari U S J Grace Felciya GF

Biotechnology reports (Amsterdam, Netherlands) 20200917

Biofilms are multi-species bacterial communities with complex structures that create antibiotic resistance, cause life-threatening infections, thereby considerable economic loss; needed new approaches. Medicinal plants are focused as new alternatives for their therapeutic and antimicrobial effects. Our present study, Azadirachta indica, Moringa oleifera, Murraya koenigii, and Psidium guajava extracts were investigated against MRSA. The preliminary antimicrobial study showed pet. et ...[more]

PMID: 32995315

Similar Datasets

Project description:BackgroundStaphylococcus aureus, a commensal bacterium, colonizes the skin and mucous membranes of approximately 30% of the human population. Apart from conventional resistance mechanisms, one of the pathogenic features of S. aureus is its ability to survive in a biofilm state on both biotic and abiotic surfaces. Due to this characteristic, S. aureus is a major cause of human infections, with Methicillin-Resistant Staphylococcus aureus (MRSA) being a significant contributor to both community-acquired and hospital-acquired infections.ResultsAnalyzing non-repetitive clinical isolates of MRSA collected from seven provinces and cities in China between 2014 and 2020, it was observed that 53.2% of the MRSA isolates exhibited varying degrees of ability to produce biofilm. The biofilm positivity rate was notably high in MRSA isolates from Guangdong, Jiangxi, and Hubei. The predominant MRSA strains collected in this study were of sequence types ST59, ST5, and ST239, with the biofilm-producing capability mainly distributed among moderate and weak biofilm producers within these ST types. Notably, certain sequence types, such as ST88, exhibited a high prevalence of strong biofilm-producing strains. The study found that SCCmec IV was the predominant type among biofilm-positive MRSA, followed by SCCmec II. Comparing strains with weak and strong biofilm production capabilities, the positive rates of the sdrD and sdrE were higher in strong biofilm producers. The genetic determinants ebp, icaA, icaB, icaC, icaD, icaR, and sdrE were associated with strong biofilm production in MRSA. Additionally, biofilm-negative MRSA isolates showed higher sensitivity rates to cefalotin (94.8%), daptomycin (94.5%), mupirocin (86.5%), teicoplanin (94.5%), fusidic acid (81.0%), and dalbavancin (94.5%) compared to biofilm-positive MRSA isolates. The biofilm positivity rate was consistently above 50% in all collected specimen types.ConclusionsMRSA strains with biofilm production capability warrant increased vigilance.

Dataset Information

Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant Staphylococcus aureus.

Publications

Investigation on the biofilm eradication potential of selected medicinal plants against methicillin-resistant <i>Staphylococcus aureus</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets