Circulating Exosomal miRNAs Signal Circadian Misalignment to Peripheral Metabolic Tissues.

ABSTRACT: Night shift work increases risk of metabolic disorders, particularly obesity and insulin resistance. While the underlying mechanisms are unknown, evidence points to misalignment of peripheral oscillators causing metabolic disturbances. A pathway conveying such misalignment may involve exosome-based intercellular communication. Fourteen volunteers were assigned to a simulated day shift (DS) or night shift (NS) condition. After 3 days on the simulated shift schedule, blood samples were collected during a 24-h constant routine protocol. Exosomes were isolated from the plasma samples from each of the blood draws. Exosomes were added to naïve differentiated adipocytes, and insulin-induced pAkt/Akt expression changes were assessed. ChIP-Seq analyses for BMAL1 protein, mRNA microarrays and exosom

SUBMITTER: Khalyfa A 

PROVIDER: S-EPMC7503323 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature