Analysis of Early Cone Dysfunction in an In Vivo Model of Rod-Cone Dystrophy.

ABSTRACT: Retinitis pigmentosa (RP) is a generic term for a group of genetic diseases characterized by loss of rod and cone photoreceptor cells. Although the genetic causes of RP frequently only affect the rod photoreceptor cells, cone photoreceptors become stressed in the absence of rods and undergo a secondary degeneration. Changes in the gene expression profile of cone photoreceptor cells are likely to occur prior to observable physiological changes. To this end, we sought to achieve greater understanding of the changes in cone photoreceptor cells early in the degeneration process of the Rho-/- mouse model. To account for gene expression changes attributed to loss of cone photoreceptor cells, we normalized PCR in the remaining number of cones to a cone cell reporter (OPN1-GFP). Gene expression pr

SUBMITTER: Hassall MM 

PROVIDER: S-EPMC7503557 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature