Characterization of the Bacteriophage vB_EfaS-271 Infecting Enterococcus faecalis.

ABSTRACT: A newly isolated bacteriophage infecting Enterococcus faecalis strains has been characterized, including determination of its molecular features. This phage, named vB_EfaS-271, has been classified as a Siphoviridae member, according to electron microscopy characterization of the virions, composed of a 50 nm-diameter head and a long, flexible, noncontractable tail (219 × 12.5 nm). Analysis of the whole dsDNA genome of this phage showed that it consists of 40,197 bp and functional modules containing genes coding for proteins that are involved in DNA replication (including DNA polymerase/primase), morphogenesis, packaging and cell lysis. Mass spectrometry analysis allowed us to identify several phage-encoded proteins. vB_EfaS-271 reveals a relatively narrow host range, as it is

SUBMITTER: Topka-Bielecka G 

PROVIDER: S-EPMC7503890 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature