High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75.

ABSTRACT: Recently an increasing number of new adenovirus types associated with type-dependent pathogenicity have been identified. However, identification of these clinical isolates represents the very first step to characterize novel pathogens. For deeper analyses, these adenoviruses need to be further characterized in basic virology experiments or they could be applied in translational research. To achieve this goal, it is essential to get genetic access and to enable genetic modification of these novel adenovirus genomes (deletion, insertion, and mutation). Here we demonstrate a high-throughput approach to get genetic access to new adenoviruses via homologous recombination. We first defined the cloning conditions regarding homology arm-length and input adenoviral genome amounts. Then we cloned fo

SUBMITTER: Zhang W 

PROVIDER: S-EPMC7504450 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature