Exosomal miR-141 promotes tumor angiogenesis via KLF12 in small cell lung cancer.

ABSTRACT:

Background

Angiogenesis, a basic requirement for tumor cell survival, is considered to be a malignant characteristic of small cell lung cancer (SCLC) and is closely related to the poor outcomes of SCLC patients. miR-141 has been found to play pro- and antiangiogenic roles in different cancers, but its role in SCLC angiogenesis has never been explored.

Methods

Total RNA was isolated from plasm exosomes and serum of SCLC patients to examine the expression of miR-141 by qRT-PCR. Cell proliferation, invasion, migration, tube formation assay, aortic ring assay and mouse tumor model were used to investigate the effect of exosomal miR-141 in angiogenesis in vitro and in vivo. Dual-luciferase assay was conducted to explore the target gene of miR-141.

Results

Circulating miR-1

SUBMITTER: Mao S 

PROVIDER: S-EPMC7504642 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature