Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.

ABSTRACT:

Background

Microglia, the primary resident myeloid cells of the brain, play critical roles in immune defense by maintaining tissue homeostasis and responding to injury or disease. However, microglial activation and dysfunction has been implicated in a number of central nervous system (CNS) disorders, thus developing tools to manipulate and replace these myeloid cells in the CNS is of therapeutic interest.

Methods

Using whole body irradiation, bone marrow transplant, and colony-stimulating factor 1 receptor inhibition, we achieve long-term and brain-wide (~ 80%) engraftment and colonization of peripheral bone marrow-derived myeloid cells (i.e., monocytes) in the brain parenchyma and evaluated the long-term effects of their colonization in the CNS.

Results

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SUBMITTER: Hohsfield LA 

PROVIDER: S-EPMC7504855 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature