In vitro endothelial hyperpermeability occurs early following traumatic hemorrhagic shock.

ABSTRACT: BACKGROUND:Endothelial hyperpermeability is suggested to play a role in the development of microcirculatory perfusion disturbances and organ failure following hemorrhagic shock, but evidence is limited. OBJECTIVE:To study the effect of plasma from traumatic hemorrhagic shock patients on in vitro endothelial barrier function. METHODS:Plasma from traumatic hemorrhagic shock patients was obtained at the emergency department (ED), the intensive care unit (ICU), 24 h after ICU admission and from controls (n = 8). Sublingual microcirculatory perfusion was measured using incident dark field videomicroscopy at matching time points. Using electric cell-substrate impedance sensing, the effects of plasma exposure on in vitro endothelial barrier function of human endothelial cells were assessed. RESUL

SUBMITTER: van Leeuwen ALI 

PROVIDER: S-EPMC7504990 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature