Phenotype-Guided Natural Products Discovery Using Cytological Profiling.

ABSTRACT: Phenotype-guided natural products discovery is emerging as a useful new discovery tool that addresses challenges in early, unbiased natural product biological annotation. These high-content approaches yield screening results that report directly on the impact of test compounds on cellular processes in target organisms and can be used to predict the modes of action of bioactive constituents from primary screening data. In this study we explored the use of our recently implemented cytological profiling platform for the isolation of compounds with a specific, predefined mode of action, namely, induction of mitotic arrest. Screening of a microbially derived extract library revealed six extracts whose cytological profiles clustered closely with those of known antimitotic agents from the pure co

SUBMITTER: Ochoa JL 

PROVIDER: S-EPMC7505087 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature