Antiplatelet Effect of Carbon Monoxide Is Mediated by NAD+ and ATP Depletion.

ABSTRACT: OBJECTIVES:Carbon monoxide (CO) produced by haem oxygenases or released by CO-releasing molecules (CORM) affords antiplatelet effects, but the mechanism involved has not been defined. Here, we tested the hypothesis that CO-induced inhibition of human platelet aggregation is mediated by modulation of platelet bioenergetics. Approach and Results: To analyze the effects of CORM-A1 on human platelet aggregation and bioenergetics, a light transmission aggregometry, Seahorse XFe technique and liquid chromatography tandem-mass spectrometry-based metabolomics were used. CORM-A1-induced inhibition of platelet aggregation was accompanied by the inhibition of mitochondrial respiration and glycolysis. Interestingly, specific inhibitors of these processes applied individually, in contrast to combined t

SUBMITTER: Kaczara P 

PROVIDER: S-EPMC7505148 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature