[National surveillance of clinical isolates of Enterococcus faecalis resistant to linezolid carrying the optrA gene in Colombia, 2014-2019].

ABSTRACT:

Objective

To describe the epidemiological, phenotypical and genetic characteristics of clinical isolates carrying the optrA gene identified in antimicrobial resistance surveillance by the laboratory of the National Institute of Health of Colombia.

Methods

Between October 2014 and February 2019, 25 isolates of Enterococcus spp. resistant to linezolid were received. Antimicrobial identification and sensitivity were determined using Vitek 2 and the minimum inhibitory concentration (MIC) to linezolid was established with E-test. The optrA gene was detected by PCR, and the genetic diversity of optrA-positive isolates was tested with Diversilab^®. Six isolates were selected to perform whole genome sequencing.

Results

The optrA ge

SUBMITTER: Saavedra SY 

PROVIDER: S-EPMC7505479 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature