A forward genetic screen in Drosophila melanogaster to identify mutations affecting INAD localization in photoreceptor cells.

ABSTRACT: In Drosophila photoreceptors, the multivalent PDZ protein INAD interacts with multiple signaling components and localizes complexes to the rhabdomere, a subcellular compartment specialized for phototransduction. Since this localization is critical for signaling, we conducted a genetic screen of the third chromosome for mutations that result in mislocalization of an INAD-GFP fusion protein. We identified seven mutant lines that fall into two complementation groups, idl (INAD localization)-A and idl-B. We show that idl-A mutants fail to complement with chaoptic (chp) mutants. Since chaoptin is a structural component of the rhabdomere, mislocalization of INAD may be a secondary effect of the retinal degeneration in chp and idl-A mutants. Genetic complementation and DNA sequencing reveal that

SUBMITTER: Sanxaridis PD 

PROVIDER: S-EPMC7505561 | biostudies-literature | 2010 Apr-Jun

REPOSITORIES: biostudies-literature