Evaluation and Optimization of Protein Extraction From E. coli by Electroporation.

ABSTRACT: Growing diversity of protein-based technologies dictates further development of bio manufacturing to lower the cost of production and maximize yields. Intracellularly expressed recombinant proteins must be extracted from production host prior to purification. Use of electroporation to obtain proteins from bacteria and yeasts has been demonstrated in several studies for different modes of operation and formats. Here we tested various protocols for protein extraction from Escherichia coli by means of electroporation. The tested protocols were compared to established extraction methods of ultrasonication and glass-bead milling in terms of protein yields and content of impurities such as host cell DNA and endotoxins in the lysate. Protein extraction yield was maximal when exponentially

SUBMITTER: Haberl Meglic S 

PROVIDER: S-EPMC7506034 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature