Serum-deprivation leads to activation-like changes in primary microglia and BV-2 cells but not astrocytes.

ABSTRACT: The aim of the present study was to determine the effect of serum deprivation on primary microglia, BV-2 cells and primary astrocytes. Cell morphology combined with the expression of phospho-(p-)38 and p-extracellular signal-regulated kinase (ERK) were assessed. Serum deprivation resulted in various alterations in the three cell cultures. Primary microglia and BV-2 cells exhibited alterations indicative of activation under serum treatment, as well as lipopolysaccharide (LPS) treatment. However, astrocytes did not react as fast. Regarding morphology, the processes present on the primary microglia and BV-2 cells became shorter and the cell bodies became larger, and more transparent vesicles were observed within the cell bodies, which indicated their increased phagocytic ability. At the prote

SUBMITTER: Yao Y 

PROVIDER: S-EPMC7506474 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature