Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye.

ABSTRACT: We have developed an alginate hydrogel-embedded capillary sensor (AHCS) for naked eye-based quantification of immunoassay. Alkaline phosphatase (ALP) can modulate gel-sol transformation to increase the permeability of Cu²⁺-cross-linked alginate hydrogel film in the AHCS, followed by solution exchange into the capillary. Through measuring the length of the liquid phase of the microfluidics in the capillary at a given time, the concentration of the ALP could be quantified with the naked eye. Since ALP is widely applied as a signal reporter for immunoassays, the AHCS could easily accommodate conventional immune sensing platforms. We justify the practicality of AHCS with hepatitis B virus surface antigen (HBsAg) in serum samples and got comparable results with commercialized immunoa

SUBMITTER: Zheng W 

PROVIDER: S-EPMC7506732 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature