MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1.

ABSTRACT:

Background/aims

MicroRNA(miR)-345-5p plays a key role in various cellular functions. However, the function of miR-345-5p in resistant depression (TRD) is unclear. The aim of this study was to evaluate the role and mechanism of miR-345-5p in the treatment of resistance depression (TRD).

Methods

RT-qPCR was used to detect the expression of miR-345-5p in BV-2 microglia. CCK-8 method and flow cytometry were used for cell viability and apoptosis of microglia. Target gene prediction and screening, and luciferase reporter assays were used to verify the downstream target gene of miR-345-5p. Western blot was used to analyze the protein expression of related proteins.

Results

miR-345-5p increased the cell viability of BV-2 microglia and the expression level of pro-inflammatory

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7507044 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature