Chromatin accessibility landscape of pediatric T-lymphoblastic leukemia and human T-cell precursors.

ABSTRACT: We aimed at identifying the developmental stage at which leukemic cells of pediatric T-ALLs are arrested and at defining leukemogenic mechanisms based on ATAC-Seq. Chromatin accessibility maps of seven developmental stages of human healthy T cells revealed progressive chromatin condensation during T-cell maturation. Developmental stages were distinguished by 2,823 signature chromatin regions with 95% accuracy. Open chromatin surrounding SAE1 was identified to best distinguish thymic developmental stages suggesting a potential role of SUMOylation in T-cell development. Deconvolution using signature regions revealed that T-ALLs, including those with mature immunophenotypes, resemble the most immature populations, which was confirmed by TF-binding motif profiles. We integrated ATAC-Seq and RN

SUBMITTER: Erarslan-Uysal B 

PROVIDER: S-EPMC7507092 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature